Anthony Kearsley, Mathematical Analysis and Modeling Group, National Institute of Standards and Technology (NIST)
Control of inward solidification in Cryobiology
For many years, mathematical models that predict a cell鈥檚 response to encroaching听ice has played an important role in developing cryopreservation protocols. It is clear听that information about the cellular state as a function of cooling rate can improve the听design of cryopreservation protocols and explain reasons for cell damage during freezing.However, previous work has ignored the interaction between the important solutes, the听effects on the state of the cell being frozen and encroaching ice fronts. In this talk, I听will survey our work on this problem and examine the cryobiologically relevant setting听of a spherically-symmetric model of a biological cell separated by a ternary fluid mixturefrom an encroaching solid鈥搇iquid interface and will illustrate our work on a simplified 1-D听problem. In particular, I will demonstrate how the thermal and chemical states inside the听cell are influenced and can potentially be controlled by altering cooling protocols at the听external boundary.
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